This year we celebrate the 10th anniversary of one of our QTOFs. It is not among the oldest mass spectrometers that we have in the lab, but it is a special case in many senses. The anniversary evoked both retrospective and prospective reflections, which I wanted to share to hear about the experience of others. The first ten years The birthday boy is an…
Imagine running a study with 100 samples by untargeted LC-MS (polar or lipids, you pick!). Data acquisition by DIA/DDA in complete in 1-2 days, and comes the step of data analysis. The minimum expectation for the software is to find all peaks across samples (rare and frequent, high and low, symmetric and tailing peaks), take care of shifts in m/z and RT, group isotopologues…
Comment: Thank you for reading this. Keep in mind that this is ALL about FIA. It's an extreme case and a special niche. Don't extrapolate any of this to LC-MS, because it's mixing apples and oranges. I'll post later on "Why do we prefer Orbitraps over TOFs for LC-MSMS", and stress critical flaws of TOF detectors and TOF data analysis. For now, happy reading…